GMP-manufactured

High-viability, high proliferation rate and well-maintained stemness

Freeze directly in -80°C freezer

FDA DMF-039214

NMPA-F20250000240

Ordering Information of Stem Cell Cryopreservation Medium

For details about the products, delivery terms or price quote, please contact us!

What is MSC Cryopreservation Medium?

MSC cryopreservation medium, also known as mesenchymal cryopreservation medium, is a specialized formulation designed for the long-term cryogenic storage of mesenchymal stem cells (MSCs) at ultra-low temperatures, typically in liquid nitrogen (-196°C). Its core objective is to preserve high post-thaw viability, efficient recovery, and essential functions — including proliferation, multi-lineage differentiation, immunomodulation, and therapeutic potency — ensuring suitability for research, regenerative medicine, and clinical applications. Traditional MSC cryopreservation medium relies on dimethyl sulfoxide (DMSO) (5–10%) as the main penetrating cryoprotectant to prevent intracellular ice formation, osmotic stress, and membrane rupture. This dmso cell culture freezing often paired with serum (e.g., FBS or human platelet lysate) or albumin for stability. However, DMSO's potential cytotoxicity — to cells during exposure and to patients during infusion — drives ongoing advancements toward DMSO-reduced or DMSO-free alternatives. These incorporate penetrating agents (e.g., glycerol, ethylene glycol) and non-penetrating protectants (e.g., trehalose, sucrose, isoleucine, polymers like PVA). Chemically defined, serum-free, animal component-free options deliver comparable or superior post-thaw performance, enhanced safety, consistency, and regulatory compliance across MSC sources (bone marrow, adipose, umbilical cord).

Key Features of MSC Cryopreservation Media

Effective mesenchymal cryopreservation media for cells and MSC cryopreservation medium are engineered to ensure safe, reliable long-term storage while meeting stringent regulatory standards for research and clinical applications. The essential features include:

Xeno-Free Composition — Completely free of animal-derived components (e.g., no fetal bovine serum or other xenogenic materials), eliminating risks of immunogenicity, zoonotic pathogen transmission, and batch-to-batch variability. This is critical for GMP-compliant, clinical-grade products and patient safety.

Chemically Defined Formulation — Composed of precisely known, synthetic ingredients with no undefined elements, ensuring reproducibility, consistency across lots, and full traceability. This supports standardized manufacturing and regulatory approval.

High Post-Thaw Viability and Recovery — Delivers excellent cell survival rates (typically >90%), rapid attachment, proliferation, and functional recovery after thawing. Optimized media preserve spindle-shaped morphology, surface markers, multipotency, and therapeutic properties like immunomodulation and differentiation potential.

Robust Cryoprotective Agents — Incorporate effective cryoprotectants to prevent ice crystal formation, osmotic injury, and membrane damage during controlled-rate freezing and thawing. Traditional options include 5–10% DMSO, but advanced media favor DMSO-reduced or fully DMSO-free alternatives for reduced toxicity, better infusion safety, and compatibility with serum-free/xeno-free workflows.

If you want to know more about tissue culture media, please visit our website.

GMP-compliant management with fully PRC Pharmacopoeia ingredients

High-viability, high proliferation rate and well-maintained stemness achieved with stem cell freezing media.

Identity of Stem Cell Cryopreservation Medium (DMSO-free)

Cryopreservation Protocol for Stem Cell DMSO Free Cryopreservation Media Digest and collect cells to be frozen,centrifuge the cell (400×g,5 minutes,RT) and remove the supernatant.

Resuspend cell pellet with proper solution (saline,culture medium or DPBS) ,followed by cell counting and cell survival rate detection.A viability of over 90% before cryopreservation is preferred.Then centrifuge (400×g,5 minutes,RT) and remove the supernatant.

Resuspend cell pellet using the product to reach a cell density of 1×10⁶-2×107/mL and a freezing volume of 0.5-1.5 mL/tube.If using a cryopreservation bag for large volume cryopreservation,please contact the technical team to obtain the programed cooling curve.Users can also add autologous plasma,serum,or HSA according to experimental needs to improve post-thaw viability.

Transfer the cryovial to a-80℃ freezer.If long-term freezing is required,transfer to liquid nitrogen after 12hours.

Thawing Protocol for Stem Cell Cryopreservation Media (DMSO-free)

1. After removing from liquid nitrogen,immediately immerse the cryovial into a 37℃ water bath,stir to rapidly thaw the cells within 2 minutes.

2. Transfer thawed cells to a conical tube,slowly and 9 times volume of proper diluent.

3. Equilibrate by gently invert the tube for 10 times.

4. Centrifuge (400×g,5minutes,RT) and remove the supernatant.

5. Resuspend cell pellet with proper media/solution.

6. Perform cell count test if needed.

7. Proceed for downstream application.

Viability Test for Cryopreservation of Stem Cells Medium (DMSO-free) Add thawed cells to 4 times the volume of complete culture medium,transfer to a centrifuge tube,and centrifuge for 5 minutes.Discard the supernatant,resuspend the cells using DPBS or complete culture medium,and then test the cell survival rate.

Ordering Information of Stem Cell Cryopreservation Medium (DMSO-free)

For details about the products, delivery terms or price quote, please contact us!

Types of Stem Cells Cryopreservation Media

Stem Cell Cryopreservation Medium For the cryopreservation of mesenchymal stem cells (MSCs) derived from various sources

Stem Cell Cryopreservation Medium (DMSO-free) For the cryopreservation of mesenchymal stem cells (MSCs) derived from various sources, such as umbilical cord and bone marrow

Neural Precursor Cell Cryopreservation Medium For the cryopreservation of neural precursor cells

Cell Cryopreservation Medium (DMSO-free) For the cryopreservation of cells such as PBMCs, stem cells and T cells

Cryopreservation of Stem Cells FAQs

Your data demonstrates high recovery rates after using the freezing media for cells. Were these results specifically observed in mesenchymal stem cells (MSCs)? Yes, our primary validation used bone marrow and umbilical cord-derived MSCs, but clients have also successfully preserved amniotic epithelial stem cells and dental pulp stem cells with comparable outcomes.

Would the cryopreservation of stem cells perform equally well for other stem cell types? While efficiency varies slightly by cell source and donor, our cryopreservation protocol consistently achieves higher viability than competitors' solutions.

Which stem cell cryopreservation protocol is recommended for adipose-derived MSCs? Cellstore Stem Cell Cryopreservation Media.

What's the maximum validated storage duration for your DMSO-free stem cell freezing media? We've confirmed viability after 12+ months in cryostorage, with no observed degradation.

Can cells truly be stored indefinitely using your stem cell cryopreservation protocol? In theory, yes. Our ice-control technology prevents long-term cryodamage, but real-world validation beyond 1-2 years remains ongoing.

What makes your stem cell freezing media chemically distinct? Does it contain antifreeze proteins? No. This cryopreservation media for cells is serum-free, xeno-free, and relies on pharmacopeia-grade salts + proprietary crystal inhibitors—no animal proteins or DMSO.

How does your stem cell cryopreservation protocol ensure clinical-grade safety? GMP-compliant manufacturing, coupled with stringent endotoxin/pyrogen testing, guarantees compliance for therapeutic applications.

GMP-manufactured with pharmacopeia ingredients

Chemically Defined

Protein-free

High-density, high-viability

Identity of PBMC Cryopreservation Protocol Medium

Cryopreservation Protocol for PBMC Freezing Media Collect the mononuclear cells from peripheral blood or cord blood,centrifuge the cell (500×g,5minutes,RT) and remove the supernatant.

Resuspend cell pellet with proper solution (saline,culture medium or DPBS) ,followed by cell counting and cell survival rate detection.A viability of over 90% before cryopreservation is preferred.Then centrifuge (500×g,5 minutes,RT) and remove the supernatant.

Resuspend cell pellet using the product to reach a cell density of 1×10⁶-1×108/mL and a freezing volume of 0.5-1.5 mL/tube.If using a cryopreservation bagforlarge volume cryopreservation,please contact the technical team to obtain the programed cooling curve.Users can also add autologous plasma,serum,or HSA according to experimental needs to improve post-thaw viability.

Transfer the cryovial to a-80℃ freezer.If long-term freezing is required,transfer to liquid nitrogen after 12hours.

Thawing Protocol for PBMC Medium

1. After removing from liquid nitrogen,immediately immerse the cryovial into a 37℃ water bath,stir to rapidly thaw the cells within 2 minutes.

2. Transfer thawed cells to a conical tube,slowly add 9 times volume of proper diluent.

3. Equilibrate by gently invert the tube for 10 times.

4. Centrifuge (500×g,5 minutes,RT) and remove the supernatant.

5. Resuspend cell pellet with proper media/solution.

6. Perform cell count test if needed.

7. Proceed for downstream application.

Viability Test for PBMC Cryopreservation Medium Add thawed cells to 4 times the volume of complete culture medium,transfer to a centrifuge tube,and centrifuge for 5 minutes.Discard the supernatant,resuspend the cells using DPBS or complete culture medium,and then test the cell survival rate.

Ordering Information of PBMC Cryopreservation Media

For details about the products, delivery terms or price quote, please contact us!

GMP-compliant management with fully PRC Pharmacopoeia ingredients

High-density, high-viability and high-killing efficiency achieved with cell culture freezing media

Ordering Information of Immune t cell freezing media

Immune t cell expansion medium

10% DMSO

100ml

CS-AT-D2

For details about the products, delivery terms or price quote, please contact us!

For more information about cryopreservation medium, please feel free to contact us!

We can also provide kinds of cryopreservation of stem cells for sale, if you are interested, please contact us.

ISO 9001, ISO 13485, ISO 45001, ISO 14001

Joint IND Submission

GMP-compliant management with fully PRC Pharmacopoeia ingredients

High-density and high-efficiency

Ordering Information of nk cell medium

NK cell culture media

10% DMSO

100ml

CS-NK-D1

For details about the products, delivery terms or price quote, please contact us!

For the cryopreservation of NK cells and IPSC-induced iNK cells from peripheral blood, umbilical cord blood and other sources

NK Cell Culture Media FAQs How is the tumor cytotoxicity assay conducted? After thawing, NK cells are cultured in NK cell cryopreservation medium for 4 hours and 12 hours, then co-incubated with target cells for 4 hours. Tumor cell death is evaluated using flow cytometry.

Has physiological saline been used to dilute NK cells post-thawing to achieve a specific cell density for viability stability testing in the NK cell culture protocol? No, but this can be arranged upon request.

Does the NK cell cryopreservation solution include DMSO, and if so, what is its approximate concentration? Both NK1 and NK3 cryopreservation solutions contain approximately 10% DMSO.

Have you tested the viability stability of NK cells in cryobags using the NK cell culture protocol? Not yet. All tests have been conducted in cryovials. We have evaluated NK cell stability in cryovials for up to 6 hours in cryopreservation solution before freezing and after thawing. For T cells, extensive cryobag testing has been performed.

Why do NK cells, cryopreserved for 2 weeks and cultured for 48 hours in NK cell culture media, show clumping and low viability (30%) despite 87% pre-freeze and 82.7% post-thaw viability? Is handling a factor? NK cells naturally have low post-thaw viability, with clumping indicating dead cells. The NK cell source and medium are key factors. Optimizing the medium with human albumin helps. Cryopreservation solution (even CS10) has minimal impact. See our PPT for client data.

A client reports that the NK3 cryopreservation solution is viscous and forms bubbles during use in the NK cell culture protocol. How can this be prevented? For freezing, use reverse pipetting (draw at second stop, dispense at first stop). Mix gently, avoid fully expelling the solution during pipetting, keep liquid in the tip, and release along the tube wall to avoid injecting directly into the suspension.

Do you have data on NK cells cultured for 24 hours post-thaw in NK cell culture media? Yes, our brochures and PPT include post-thaw culture data at 12, 24, 36, and 48 hours.

Is there high-density cryopreservation data for NK cells? Internal tests show viability at 2E8 and 5E8, but we recommend ≤2E8 for clients. Some clients successfully use 2E8.

What is the longest duration for which NK cell cryopreservation data is available? Data are available for up to 12 months.

Has the viability of NK cells been tested after 1 month at -80°C in NK cell medium? Yes.

Does Cellstore currently offer DMSO-free cryopreservation solutions for NK cells in the NK cell culture protocol? Not yet. Current DMSO-free prototypes achieve >90% recovery but lack sufficient tumor cytotoxicity.

For more information about cryopreservation media for cells and nk cell culture protocol, please feel free to contact us!

We can also provide kinds of freezing media for cells for sale, if you are interested, please contact us.

No clumping of cells

Higher viability

One-step washing for convenient operation

This freezing medium features optimal storage conditions for efficient transportation

Ordering Information of cell culture media storage and Transportation Solution Cell Storage and Transportation Solution DMSO-Free 500ml CS-CP-N1

For details about the products, delivery terms or price quote, please contact us!

For more information about cell cryopreservation medium and Cell storage media, please feel free to contact us!

We can also provide kinds of cryopreservation media for sale, if you are interested, please contact us.

CellStore's ice-controlling cryopreservation technology fundamentally differs from conventional methods which enable:

Ice control efficiency is tens of thousands of times that of traditional CPAs, allowing ice crystal formation and efficiently inhibiting ice crystal growth

All ice-control materials are pharmacopeia-sourced, ensuring compositional safety. They deliver high-efficiency ice-control functionality at sub-toxic dosage levels

Customized formulations via proprietary 10,000+ ice control material library

These ice-control materials can function as stand-alone cryoprotectants for safe preservation or synergize with vitrification agents to reduce their cytotoxicity — delivering a twofold enhancement in cryopreservation performance

For more information about cryopreservation media for cells and cell freezing media protocol, please feel free to contact us!

If you are looking for a reliable tissue culture media manufacturer, please contact us.